The latest reviewed version was checked on 4 August 2016. There are 4 pending changes awaiting review. Just as with applications of ion exchange chromatography pdf forms of chromatography, ion exchange chromatography utilizes both a stationary and mobile phase.
The stationary phase in this method carries either a positive or negative charge. The charged stationary phases are named according to the types of charged particles that bind to them. Negatively charged species are attracted to the resin and travel more slowly down the column. Similarly, positively charged species are attracted to a cation exchange resin’s negatively charged functional groups.
Sulfonate groups are commonly found as side-chains in cation-exchange resins. The particles that bind to the stationary phase can be released by changing the buffer conditions. The charge on the protein affects its behavior in ion exchange chromatography.